REDUCTION IN PLASMA-MEMBRANE ATPASE ACTIVITY OF TOMATO ROOTS BY SALT STRESS

被引:46
作者
GRONWALD, JW
SUHAYDA, CG
TAL, M
SHANNON, MC
机构
[1] US SALIN LAB, 4500 GLENWOOD DR, RIVERSIDE, CA 92501 USA
[2] BEN GURION UNIV NEGEV, DEPT BOT, IL-84120 BEER SHEVA, ISRAEL
[3] UNIV MINNESOTA, DEPT AGRON & RANGE SCI, USDA ARS, PLANT SCI RES UNIT, ST PAUL, MN 55108 USA
关键词
ATPase; plasma membrane; salinity; salt-stress;
D O I
10.1016/0168-9452(90)90198-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ATPase activity was characterized in a plasma membrane-enriched fraction isolated from tomato (Lycopersicon exculentum Mill., cv. Heinz 1350) roots grown in the absence or presence of salinity stress (-4 bars, 60 mM NaCl plus 12 mM CaCl2). The enzyme exhibited the following properties in both non-stressed and salt-stressed roots: (1) activated by divalent cations (Mg2+ ≻ Mn2+ ≻ Co2+ ≻ Ni2+ ≻ Ca2+ ≻ Zn2+) and further stimulated by monovalent cations (Na+ = K+ ≻ Rb+ ≻ Li+); (2) pH optima for Mg2+ activation and KCl-stimulation of 7.0 and 6.5, respectively; (3) selective for Mg2+-ATP as substrate; (4) sensitive to N,N′-dicyclohexylcarbodiimide and vanadate but insensitive to azide and oligomycin; (5) not stimulated synergistically by Na+ plus K+. Exposing roots to salt-stress altered the kinetics of Mg2+-ATPase activity. Simple Michaelis-Menten kinetics were observed when Mg2+-ATP was used as substrate for both control and salt-treated roots. Salt-stress had little effect on the apparent Km for Mg2+-ATP. The predominant effect of salt-stress was reduce Vmax of Mg2+-ATPase activity from 69 μmol Pi (mg protein)-1 h-1 in control roots to 39 μmol Pi (mg protein) -1 h-1 in salt-treated roots. © 1990.
引用
收藏
页码:145 / 153
页数:9
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