EFFECT OF THE ANTITUMOR PROTEIN ALPHA-SARCIN ON THE THERMOTROPIC BEHAVIOR OF ACID PHOSPHOLIPID-VESICLES

被引:33
作者
GASSET, M
ONADERRA, M
DELPOZO, AM
SCHIAVO, GP
LAYNEZ, J
USOBIAGA, P
GAVILANES, JG
机构
[1] UNIV COMPLUTENSE MADRID,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-28040 MADRID,SPAIN
[2] CSIC,INST QUIM FIS ROCASOLANO,MADRID 6,SPAIN
[3] UNIV PADUA,IST PATOL GEN,I-35100 PADUA,ITALY
[4] CTR CONSIGLIO NAZL RIC BIOMEMBRANE,PADUA,ITALY
关键词
ANTITUMOR AGENT; THERMOTROPIC BEHAVIOR; PHOSPHOLIPID VESICLE; ALPHA-SARCIN;
D O I
10.1016/0005-2736(91)90055-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The antitumour protein alpha-sarcin modifies the thermotropic behaviour of phospholipid vesicles. This has been studied by fluorescence depolarization measurements and differential scanning calorimetry. A surface protein-phospholipid interaction is detected by measuring the polarization degree of TMA-DPH-labelled vesicles. At the higher protein/lipid molar ratios studied, the alpha-sarcin-vesicles complexes exhibit different thermotropic behaviour depending on whether they are prepared above or below the T(m) of the corresponding phospholipid. Labelling of the protein with photoactive phospholipids has also been considered. Alpha-sarcin penetrates the bilayer deep enough to be labelled with the photoactive group located at the C-12 of the fatty acid acyl chain of phospholipids forming vesicles.
引用
收藏
页码:9 / 16
页数:8
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