CLONING AND SEQUENCING OF AN ESCHERICHIA-COLI K12 GENE WHICH ENCODES A POLYPEPTIDE HAVING SIMILARITY TO THE HUMAN FERRITIN H-SUBUNIT

被引：51

作者：

IZUHARA, M ^{[1
]}

TAKAMUNE, K ^{[1
]}

TAKATA, R ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC SURG,INST ZOOL,SAPPORO,HOKKAIDO 060,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 225卷 / 03期

关键词：

GENOME LIBRARIES; TRANSCRIPT ANALYSIS; RNASE-III; ANCIENT PROTEIN;

D O I：

10.1007/BF00261694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using lambda phage clones containing segments of the Escherichia coli K12 chromosome as hybridization probes, we found one gene at 42 min on the E. coli chromosome map, the expression of which was affected by RNase III. The sequence of the DNA fragment containing this gene (gen-165) revealed the presence of an open reading frame encoding a polypeptide of 165 amino acid residues. The amino acid sequence deduced from the nucleotide sequence exhibited a remarkable similarity to that of the human ferritin H chain.

引用

页码：510 / 513

页数：4