EFFECTS OF XIMOPROFEN AND ACETYLSALICYLIC-ACID ON HUMAN ARTICULAR CHONDROCYTES IN 3-DIMENSIONAL CULTURE

被引：3

作者：

BASSLEER, C

HENROTIN, Y

FRANCHIMONT, P

机构：

[1] Department of Rheumatology, Laboratory of Radioimmunoassay, Pathology Institute, University of Liège, Liège, B-4000, Sart-Tilman par

来源：

DRUG INVESTIGATION | 1993年 / 5卷 / 01期

关键词：

D O I：

10.1007/BF03259222

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Different pharmacological concentrations of 2 nonsteroidal anti-inflammatory drugs (NSAIDs), acetylsalicylic acid (ASA) and ximoprofen (XP), were tested on human chondrocytes cultivated in clusters. DNA synthesis, measured by [H-3]thymidine uptake into DNA, was depressed by ASA at concentrations typical of those found in synovial fluid and plasma after the administration of a single oral dose (500mg). Similarly, proteoglycans, assayed by a specific radioimmunoassay, were depressed in culture medium, whereas type II collagen was not modified. In contrast, XP did not affect these chondroformative processes in chondrocytes. Both NSAIDs were potent inhibitors of prostaglandin E2 (PGE2) synthesis, XP being more efficient than ASA. These experiments demonstrated that although XP inhibits PGE2 synthesis, it does not depress chondroformative parameters in human cartilage in vitro.

引用

页码：11 / 18

页数：8

共 31 条

[11]

Gysen P., Franchimont P., Radioimmunoassay of proteoglycans, Journal of Immunoassay, 5, pp. 221-243, (1984)

[12]

Henrotin Y., Bassleer C., Nusgens B., Franchimont P., Radioimmunoassay for human type II collagen, Journal of Immunoassay, 11, pp. 555-579, (1990)

[13]

Herbage D., Bouillet J., Bernengo J.C., Biochemical and physico-chemical characterization of pepsin-solubilized type II collagen from bovine articular cartilage, Biochemistry Journal, 161, pp. 303-312, (1977)

[14]

Hucker H.B., Stauffer S.C., White S.D., Rhodes R.E., Arisoa B.H., Et al., Physiologic disposition and metabolic fate of a new antiinflammatory agent, cis-5-fluoro-2-methyl-l-methylsulfin benzylidenyl-indende-3-acetate acid in the rat, dog, rhesus monkey and man, Drug Metabolism and Disposition, 1, pp. 721-736, (1973)

[15]

Kirkpatrick C., Mohr W., Wildfeuer A., Haferkamp O., Influence of non steroidal antiinflammatory agents on lapine articular chondrocytes growth in vitro, Rheumatology, 42, pp. 58-65, (1983)

[16]

Lequesne M., Arthrose de la hanche et du genou. Critères de diagnostic. Indices de la douleur, de la fonction et du résultat thérapeutique, Epidemiology of osteoarthritis, (1984)

[17]

Labarca C., Paigen K., A simple, rapid and sensitive DNA assay procedure, Analytical Biochemistry, 102, pp. 344-352, (1980)

[18]

Levy G., Pharmacokinetics of salicylate in man, Drug Metabolism Reviews, 9, pp. 3-19, (1979)

[19]

Mitrovic D., McCall E., Dray F., The in vitro production of prostanoids by cultured bovine articular chondrocytes, Prostaglandins, 23, pp. 17-28, (1982)

[20]

Mitrovic D., McCall E., Front D., Aprice F., Darmon N., Et al., Antiinflammatory drug, prostanoids and proteoglycan production by cultured bovine articular chondrocytes, Prostaglandins, 28, pp. 417-434, (1984)

← 1 2 3 4 →