ALTERED MEMBRANE ANISOTROPY GRADIENTS OF PLASMA-MEMBRANES OF LIVING PERIPHERAL-BLOOD LEUKOCYTES IN AGING AND ALZHEIMERS-DISEASE

被引:13
作者
COLLINS, JM
SCOTT, RB
MCCLISH, DK
TAYLOR, JR
GROGAN, WM
机构
[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT INTERNAL MED,RICHMOND,VA 23298
[2] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOSTAT,RICHMOND,VA 23298
[3] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT NEUROL,RICHMOND,VA 23298
关键词
ALZHEIMERS DISEASE; MEMBRANE RIGIDITY; PERIPHERAL BLOOD; LEUKOCYTES; FLOW CYTOMETRY;
D O I
10.1016/0047-6374(91)90081-A
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Several reports have suggested that membrane rigidity, a term that refers to the Alzheimer's Disease. Accordingly, a series of fluorescent membrane probes was used to evaluate the rigidity from the surface to the center of the outer hemi-leaflet of the plasma membrane of living neutrophils, monocytes and lymphocytes. Anisotropy, a parameter which increases with increasing membrane rigidity, was calculated from flow cytometric measurements of vertically and horizontally polarized components of the fluorescence emission of the probes. These preliminary experiments suggest that whereas membrane rigidity in certain regions of the plasma membrane of peripheral blood leukocytes is increased as expected in elderly controls, it is decreased in Alzheimer's disease.
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页码:153 / 162
页数:10
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