NUCLEOLAR TRANSCRIPTIONAL ACTIVITY IN MOUSE SERTOLI CELLS IS DEPENDENT ON CENTROMERE ARRANGEMENT

被引：41

作者：

HAAF, T ^{[1
]}

STEINLEIN, C ^{[1
]}

SCHMID, M ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT GENET,STANFORD,CA 94305

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 191卷 / 01期

关键词：

D O I：

10.1016/0014-4827(90)90051-B

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Experimental evidence suggests that centromere arrangement is relevant to the expression of ribosomal genes in murine Sertoli cells. Nuclei endowed with a nucleolus inactive in rRNA synthesis presented several clusters, each containing a bunch of individual centromeres. RNA polymerase I was not cytochemically detected in the nucleolar structure, which contained only small amounts of fibrillarin. In the course of nucleolar activation, the centromeres within the separate clusters became fused into larger centromeric bodies. Synthesis of precursor rRNAs and their processing were visualized by strong nucleolar fluorescence signals using antibodies to RNA polymerase I and fibrillarin. © 1990.

引用

页码：157 / 160

页数：4

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