CALCIUM IONOPHORE PLUS EXCISION INDUCE A LARGE CONDUCTANCE CHLORIDE CHANNEL IN MEMBRANE PATCHES OF HUMAN COLON-CARCINOMA CELLS HT-29CL.19A

被引：11

作者：

BAJNATH, RB

GROOT, JA

DEJONGE, HR

KANSEN, M

BIJMAN, J

机构：

[1] ERASMUS UNIV,DEPT BIOCHEM 1,3000 DR ROTTERDAM,NETHERLANDS

[2] ERASMUS UNIV,DEPT CELL BIOL,3000 DR ROTTERDAM,NETHERLANDS

来源：

EXPERIENTIA | 1993年 / 49卷 / 04期

关键词：

MAXI-CHLORIDE CHANNEL; HT-29; INTESTINAL EPITHELIUM; ELECTROLYTE TRANSPORT; CA2+-IONOPHORE;

D O I：

10.1007/BF01923409

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In excised inside-out membrane patches of the human colon carcinoma HT-29cl.19A cells a large conductance (373 +/- 10 pS) chloride channel was found. Channel activity could only be observed after excision of patches from cells incubated with calcium ionophore. The channel was never observed in cell-attached patches. The channel was strongly voltage dependent, being open only between +30 and -30 mV clamp potentials. The selectivity sequence among anions, deduced from reversal potentials, was I > Br > Cl > F > gluconate. The P(Na)/P(Cl) was 0.09. Although a similar type of channel has been described earlier, this is the first report stating its appearance in patches of intestinal epithelial cells requiring the combined action of Ca2+ ionophore and excision, suggesting its control by an intracellular compound.

引用

页码：313 / 316

页数：4

共 23 条

[11]

HANRAHAN JW, 1984, BIOPHYS J, V45, pA300

[12]

HAZAMA A, 1992, PFLUGERS ARCH, V420, pR242

[13] EXPRESSION OF THE CYSTIC-FIBROSIS GENE IN NONEPITHELIAL INVERTEBRATE CELLS PRODUCES A REGULATED ANION CONDUCTANCE [J].