INVIVO TOXICITY OF 3'-AZIDO-3'-DEOXYTHYMIDINE (AZT) ON CBA/CA MICE

被引：27

作者：

CRONKITE, EP

BULLIS, J

机构：

[1] Medical Department, Brookhaven National Laboratory, Upton, New York

[2] Medical Department, Brookhaven National Laboratory, Upton, New York

来源：

INTERNATIONAL JOURNAL OF CELL CLONING | 1990年 / 8卷 / 05期

关键词：

AIDS; AZT; Hemopoietic depression; Stem cells;

D O I：

10.1002/stem.5530080502

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

CBA/Ca male mice were given 3′‐azido‐3′‐deoxythymidine (AZT) in drinking water (1 mg/ml) for up to 7 weeks. Water consumption and body weight decreased significantly. Neutropenia and lymphopenia were observed during and after exposure. Significant macrocytic anemia developed and disappeared as a function of red cell life span after stopping AZT intake. A microthrombocytosis was seen. Bone marrow cellularity and spleen colony‐forming unit (CFU‐s) content fell, but recovered completely and quickly after terminating AZT intake. Hemopoietic stem cell function measured by 2 different methods of rescuing fatally irradiated mice was normal 4 weeks after AZT exposure, suggesting that AZT treatment does not induce a long‐lasting effect in genetic control of mitotic potential of stem cells. This is in marked contrast to exposure of CBA/Ca mice to benzene and ionizing radiation. Copyright © 1990 AlphaMed Press

引用

页码：332 / 345

页数：14

共 19 条

[1]

ABRAHAM NG, 1989, BLOOD, V74, P139

[2]

BHALLA K, 1989, BLOOD, V74, P1923

[3]

BOGLIOLO G, 1988, EXP HEMATOL, V16, P938

[4] AZIDOTHYMIDINE - CRYSTAL-STRUCTURE AND POSSIBLE FUNCTIONAL-ROLE OF THE AZIDO GROUP [J].

CAMERMAN, A ;

MASTROPAOLO, D ;

CAMERMAN, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8239-8242

[5]

CRONKITE EP, 1989, ENVIRON HEALTH PERSP, V82, P97

[6] SUPPRESSION OF INVITRO HEMATOPOIESIS FOLLOWING HUMAN IMMUNODEFICIENCY VIRUS-INFECTION [J].

DONAHUE, RE ;

JOHNSON, MM ;

ZON, LI ;

CLARK, SC ;

GROOPMAN, JE .

NATURE, 1987, 326 (6109) :200-203

[7] PHOSPHORYLATION OF 3'-AZIDO-3'-DEOXYTHYMIDINE AND SELECTIVE INTERACTION OF THE 5'-TRIPHOSPHATE WITH HUMAN-IMMUNODEFICIENCY-VIRUS REVERSE-TRANSCRIPTASE [J].

FURMAN, PA ;

FYFE, JA ;

STCLAIR, MH ;

WEINHOLD, K ;

RIDEOUT, JL ;

FREEMAN, GA ;

LEHRMAN, SN ;

BOLOGNESI, DP ;

BRODER, S ;

MITSUYA, H ;

BARRY, DW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8333-8337

[8]

GALLICCHIO VS, 1989, P SOC EXP BIOL MED, V191, P201

[9]

GANSER A, 1989, EXP HEMATOL, V17, P321

[10]

LEVIN J, 1983, J LAB CLIN MED, V101, P295

← 1 2 →