THE ROLE OF O-2-CENTER-DOT- IN THE PRODUCTION OF HO-CENTER-DOT - IN-VITRO AND IN-VIVO

In vitro O2.- reduces Fe(III) to Fe(II), which, in turn, reduces the H2O2, yielding Fe(II)O or HO.. In vivo O2.-increases the supply of free iron by oxidatively attacking the [4Fe-4S] clusters of dehydratases such that they release Fe(II), which can then reduce H2O2. In vivo. O2.- also increases the production of H2O2 by acting as an oxidant toward the dehydratases and toward other cellular reductants.