PROPERTIES OF OMEGA-CONOTOXIN MVIIC RECEPTORS ASSOCIATED WITH ALPHA-1A CALCIUM-CHANNEL SUBUNITS IN RAT-BRAIN

被引：32

作者：

MARTINMOUTOT, N ^{[1
]}

LEVEQUE, C ^{[1
]}

SATO, K ^{[1
]}

KATO, R ^{[1
]}

TAKAHASHI, M ^{[1
]}

SEAGAR, M ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN

来源：

FEBS LETTERS | 1995年 / 366卷 / 01期

关键词：

CALCIUM CHANNEL; OMEGA CONOTOXIN MVIIC RECEPTOR; ALPHA-1A SUBUNIT; ALPHA-2; SUBUNIT;

D O I：

10.1016/0014-5793(95)00467-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Solubilized I-125-omega conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel alpha 1A subunit. Anti-alpha 1A antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked I-125-omega conotoxin MVIIC to an alpha 2 delta-like 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity omega conotoxin MVIIC binding sites formed by alpha 1A subunits are located in close proximity to peripheral alpha 2 subunits.

引用

页码：21 / 25

页数：5

共 25 条

[1] TOXITYPING RAT-BRAIN CALCIUM CHANNELS WITH OMEGA-TOXINS FROM SPIDER AND CONE SNAIL VENOMS [J].

ADAMS, ME ;

MYERS, RA ;

IMPERIAL, JS ;

OLIVERA, BM .

BIOCHEMISTRY, 1993, 32 (47) :12566-12570

[2] PROPERTIES OF STRUCTURE AND INTERACTION OF THE RECEPTOR FOR RHO-CONOTOXIN, A POLYPEPTIDE ACTIVE ON CA-2+ CHANNELS [J].

BARHANIN, J ;

SCHMID, A ;

LAZDUNSKI, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 150 (03) :1051-1062

[3] THE NAMING OF VOLTAGE-GATED CALCIUM CHANNELS [J].

BIRNBAUMER, L ;

CAMPBELL, KP ;

CATTERALL, WA ;

HARPOLD, MM ;

HOFMANN, F ;

HORNE, WA ;

MORI, Y ;

SCHWARTZ, A ;

SNUTCH, TP ;

TANABE, T ;

TSIEN, RW .

NEURON, 1994, 13 (03) :505-506

[4]

DEJONGH KS, 1990, J BIOL CHEM, V265, P14738

[5] MOLECULAR-CLONING OF THE ALPHA-1 SUBUNIT OF AN OMEGA-CONOTOXIN-SENSITIVE CALCIUM-CHANNEL [J].

DUBEL, SJ ;

STARR, TVB ;

HELL, J ;

AHLIJANIAN, MK ;

ENYEART, JJ ;

CATTERALL, WA ;

SNUTCH, TP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :5058-5062

[6] A NEW CONUS PEPTIDE LIGAND FOR MAMMALIAN PRESYNAPTIC CA2+ CHANNELS [J].

HILLYARD, DR ;

MONJE, VD ;

MINTZ, IM ;

BEAN, BP ;

NADASDI, L ;

RAMACHANDRAN, J ;

MILJANICH, G ;

AZIMIZOONOOZ, A ;

MCINTOSH, JM ;

CRUZ, LJ ;

IMPERIAL, JS ;

OLIVERA, BM .

NEURON, 1992, 9 (01) :69-77

[7] RAT-BRAIN EXPRESSES AN ALTERNATIVELY SPLICED FORM OF THE DIHYDROPYRIDINE-SENSITIVE L-TYPE CALCIUM-CHANNEL ALPHA-2 SUBUNIT [J].

KIM, HL ;

KIM, H ;

LEE, P ;

KING, RG ;

CHIN, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3251-3255

[8] THE SYNAPTIC VESICLE PROTEIN SYNAPTOTAGMIN ASSOCIATES WITH CALCIUM CHANNELS AND IS A PUTATIVE LAMBERT-EATON MYASTHENIC SYNDROME ANTIGEN [J].

LEVEQUE, C ;

HOSHINO, T ;

DAVID, P ;

SHOJIKASAI, Y ;

LEYS, K ;

OMORI, A ;

LANG, B ;

ELFAR, O ;

SATO, K ;

MARTINMOUTOT, N ;

NEWSOMDAVIS, J ;

TAKAHASHI, M ;

SEAGAR, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3625-3629

[9]

LEVEQUE C, 1994, J BIOL CHEM, V269, P6306

[10]

MARQUEZE B, 1988, MOL PHARMACOL, V34, P87

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