DIRECT AUTOMATED SEQUENCING OF 16S RDNA AMPLIFIED BY POLYMERASE CHAIN-REACTION FROM BACTERIAL CULTURES WITHOUT DNA PURIFICATION

The 16S rRNA gene from various bacterial cultures was amplified by the polymerase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.