REVISIT OF MALDI FOR SMALL PROTEINS

被引：49

作者：

ZHU, YF ^{[1
]}

LEE, KL ^{[1
]}

TANG, K ^{[1
]}

ALLMAN, SL ^{[1
]}

TARANENCKO, NI ^{[1
]}

CHEN, CH ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV HLTH SCI RES,OAK RIDGE,TN 37831

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 13期

关键词：

D O I：

10.1002/rcm.1290091318

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization (MALDI) was used for several small proteins (such as insulin) and for peptides. It was found that the detection efficiencies of MALDI for the insulin B chain and the insulin A chain are drastically different. Similar phenomena were also observed for various types of peptides, The positive-ion signal of MALDI in detecting proteins or peptides was found to be greatly enhanced by the presence of a basic amino acid in their chains, The experimental results indicate that this enhancement may arise from proton transfer in solution by an acid-base reaction between the protein/peptide and matrix molecule. This pre-protonated mechanism provides a low energy barrier for the ionization of peptides in a MALDI process, and greatly reduces the energy threshold of MALDI. Matrix effects on the ionization mechanism are discussed.

引用

页码：1315 / 1320

页数：6

共 20 条

[1]

Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P436, DOI 10.1002/rcm.1290031208

[2]

Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P432, DOI 10.1002/rcm.1290031207

[3] HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY [J].