DIFFERENTIAL-EFFECTS OF EXPERIMENTALLY-INDUCED CHRONIC-PANCREATITIS ON NEUROPEPTIDE IMMUNOREACTIVITIES IN THE FELINE PANCREAS

被引:13
作者
DEGIORGIO, R
STERNINI, C
WIDDISON, AL
ALVAREZ, C
BRECHA, NC
REBER, HA
GO, VLW
机构
[1] UNIV CALIF LOS ANGELES, VAMC,SCH MED,CTR ULCER RES & EDUC,CTR DIGEST DIS, BLDG 115,ROOM 203, LOS ANGELES, CA 90073 USA
[2] UNIV CALIF LOS ANGELES, VAMC, SCH MED, DEPT MED, LOS ANGELES, CA USA
[3] UNIV CALIF LOS ANGELES, VAMC, SCH MED, DEPT ANAT & CELL BIOL, LOS ANGELES, CA USA
[4] UNIV CALIF LOS ANGELES, VAMC, SCH MED, BRAIN RES INST, LOS ANGELES, CA USA
[5] UNIV CALIF LOS ANGELES, VAMC, SCH MED, DEPT SURG, LOS ANGELES, CA USA
[6] SEPULVEDA, LOS ANGELES, CA USA
关键词
D O I
10.1097/00006676-199311000-00006
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The distribution and concentration of calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), and gastrin-releasing peptide (GRP) immunoreactivities in the pancreas of cats with experimentally induced chronic pancreatitis and of age- and sex-matched controls were investigated. By narrowing the main pancreatic duct between the head and the body to approximately 25% of its normal diameter, we induced within 5 weeks chronic pancreatitis restricted to the body and tail. In control animals, peptide immunoreactive nerves were distributed to the islets, acini, and ducts; the latter were predominantly innervated by fibers immunoreactive for NPY, VIP, or CGRP. The vasculature received an abundant supply of NPY-, CGRP-, and, to a lesser extent, SP-containing axons. Within intrapancreatic ganglia, peptide immunoreactivities were identified in fibers and ganglion cells, with the exception of CGRP and SP immunostaining, which could be visualized only in fibers. In animals with chronic pancreatitis, the innervation pattern of each peptidergic system was comparable to that described in controls. However, there was a remarkable increase in the density and staining intensity of VIP and NPY immunoreactive fibers in the exocrine parenchyma and fibrous septa of the body and tail, where chronic pancreatitis developed. Fibers immunoreactive for CGRP and SP also were moderately denser than in controls, whereas those containing GRP immunoreactivity did not show any detectable changes. In addition, a marked increase of the immunostaining for VIP and, to a much lesser extent, for NPY and GRP, was observed in neurites supplying the head of the pancreas, which appeared devoid of histologically detectable pathological alterations. Radioimmunoassay analysis confirmed the immunohistochemical observations. The increased density of distinct peptidergic nerves in the pancreas with induced chronic pancreatitis might be the result of compensatory phenomena in response to the inflammatory process.
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页码:700 / 710
页数:11
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