2 AMIDOPHOSPHORIBOSYLTRANSFERASE GENES OF ARABIDOPSIS-THALIANA EXPRESSED IN DIFFERENT ORGANS

被引：23

作者：

ITO, T

SHIRAISHI, H

OKADA, K

SHIMURA, Y

机构：

[1] NATL INST BASIC BIOL,DIV GENE EXPRESS & REGULAT 1,OKAZAKI,AICHI 444,JAPAN

[2] KYOTO UNIV,FAC SCI,DEPT BIOPHYS,KYOTO 606,JAPAN

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 01期

关键词：

AMIDOPHOSPHORIBOSYLTRANSFERASE (ATASE- EC 2.4.2.14); ARABIDOPSIS THALIANA; COLD PLAQUE SCREENING; PURINE NUCLEOTIDE BIOSYNTHESIS;

D O I：

10.1007/BF00039565

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amidophosphoribosyltransferase (ATase: EC 2.4.2.14) is a key enzyme in the pathway of purine nucleotide biosynthesis. We have identified several cDNA clones whose amino acid sequences exhibit similarity with the known ATases in a cDNA library of young floral buds of Arabidopsis thaliana. The cDNA clones are derived from two genes homologous with each other. These cDNAs represent the first plant representatives of ATase gene. Structural comparison with ATases of other organisms has revealed that the two genes encode [4Fe-4S] cluster-dependent ATases. Northern blot analysis showed that expression level of the genes is different in three organs; one gene is expressed in flowers and roots, while the other gene is mainly expressed in leaves.

引用

页码：529 / 533

页数：5

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