MODIFIED TRIPLE-RESONANCE NMR EXPERIMENTS WITH OPTIMIZED SENSITIVITY FOR RAPIDLY EXCHANGING PROTONS

被引:6
作者
JAHNKE, W [1 ]
KESSLER, H [1 ]
机构
[1] TECH UNIV MUNICH,INST ORGAN CHEM & BIOCHEM,D-85747 GARCHING,GERMANY
来源
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1995年 / 34卷 / 04期
关键词
NMR SPECTROSCOPY; STRUCTURE ELUCIDATION; PROTEINS;
D O I
10.1002/anie.199504691
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Fast exchange with surrounding water protons need not attenuate 1H NMR signals of proteins in solution. A modified pulse sequence has been developed to increase the signal intensity of the amide protons; on the right the improvement in sensitivity (a → c) is shown for a selected residue in a protein. This new technique should aid elucidation of protein structures in solution. (Figure Presented.) Copyright © 1995 by VCH Verlagsgesellschaft mbH, Germany
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页码:469 / 471
页数:3
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