MOLECULAR-CLONING OF NPY-Y1 RECEPTOR CDNA FROM RAT SPLENIC LYMPHOCYTES - EVIDENCE OF LOW-LEVELS OF MESSENGER-RNA EXPRESSION AND [I-125] NPY BINDING-SITES

被引：52

作者：

PETITTO, JM

HUANG, Z

MCCARTHY, DB

机构：

[1] UNIV FLORIDA, COLL MED, DEPT PHARMACOL, GAINESVILLE, FL 32610 USA

[2] VET ADM MED CTR, GAINESVILLE, FL 32610 USA

[3] UNIV FLORIDA, COLL MED, DEPT NEUROSCI, GAINESVILLE, FL 32610 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1994年 / 54卷 / 1-2期

关键词：

CELLULAR IMMUNE; NEUROPEPTIDE Y RECEPTORS; CLONING; GENE EXPRESSION; RADIOLIGAND RECEPTOR BINDING;

D O I：

10.1016/0165-5728(94)90234-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study addressed the question of whether the effects of neuropeptide Y(NPY) on parameters of cellular immune activity are mediated by the direct action of this neuropeptide on lymphocyte NPY receptors. A partial cDNA corresponding to bp 3-585 of the NPY-Y1 receptor coding sequence was cloned from rat splenic lymphocytes and found to have 100% nucleotide sequence homology with that segment of the NPY-Y1 receptor in brain. Basal levels of NPY-Y1 mRNA expression and [I-125]NPY binding sites of rat splenic lymphocytes were markedly lower than in frontal cortex. These data provide the first direct evidence that cells of the immune system possess NPY receptors, and suggest that further study will be necessary to determine their physiological significance.

引用

页码：81 / 86

页数：6

共 33 条

[31] NEUROPEPTIDE-Y-RELATED PEPTIDES AND THEIR RECEPTORS - ARE THE RECEPTORS POTENTIAL THERAPEUTIC DRUG TARGETS [J].