MOLECULAR ANALYSIS OF MUTATIONS INDUCED BY 2-CHLOROACETALDEHYDE, THE ULTIMATE CARCINOGENIC FORM OF VINYL-CHLORIDE, IN HUMAN-CELLS USING SHUTTLE VECTORS

被引：48

作者：

MATSUDA, T

YAGI, T

KAWANISHI, M

MATSUI, S

TAKEBE, H

机构：

[1] KYOTO UNIV,FAC MED,DEPT RADIAT GENET,SAKYO KU,KYOTO 60601,JAPAN

[2] KYOTO UNIV,CTR ENVIRONM QUAL CONTROL,OTSU,SHIGA 520,JAPAN

来源：

CARCINOGENESIS | 1995年 / 16卷 / 10期

关键词：

D O I：

10.1093/carcin/16.10.2389

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Vinyl chloride (VC) is a carcinogen associated with human and animal cancers. The ultimate carcinogenic form of VC, 2-chloroacetaldehyde (CAA), has been suspected to be mutagenic and we confirmed the mutagenicity of CAA using a modified shuttle vector plasmid. Base sequence analyses of 109 mutant plasmids with mutations in the supF gene, which were treated with CAA and propagated in the cultured human cells, revealed that more than half of the single base substitutions were G:C to A:T transitions with eight hotspots. The majority of the mutations involving G:C base pairs were in 5'-AAGG-3' or 5'-CCTT-3' sequences suggesting that these sequences are the main targets of mutagenesis caused by CAA.

引用

页码：2389 / 2394

页数：6

共 42 条

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