AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE ANTINEOPLASTIC AGENT VINCRISTINE

被引：13

作者：

LAPINJOKI, SP ^{[1
]}

VERAJANKORVA, HM ^{[1
]}

HUHTIKANGAS, AE ^{[1
]}

LEHTOLA, TJ ^{[1
]}

LOUNASMAA, M ^{[1
]}

机构：

[1] TECH UNIV HELSINKI, DEPT CHEM, SF-02150 ESPOO, FINLAND

来源：

JOURNAL OF IMMUNOASSAY | 1986年 / 7卷 / 1-2期

关键词：

D O I：

10.1080/01971528608063049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme-linked immunosorbent assay for vincristine was developed, based on a new procedure for synthesizing the hapten-protein conjugate. In both the immunogen and the enzyme tracer a spacer group was introduced between the hapten and protein, and the vincristine was coupled at a site far from its functional groups. The antibody produced proved to be exceptionally specific as compared with previous immunoassays for bis-indole alkaloids. Thousandfold antibody dilutions could be used and samples at the femtomole range were assayable. Applications of the method to patient plasma samples and to plant material are described.

引用

页码：113 / 128

页数：16

共 21 条

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