TANNIC-ACID STAINING AND EXTRACTION OF ENZYMES IN POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：11

作者：

AOKI, K

KAJIWARA, S

SHINKE, R

NISHIRA, H

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90775-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure was developed for a rapid staining of proteins in polyacrylamide gels with tannic acid and the extraction of enzymatic activity from the gels. Lysozyme and Taka-amylase A were stained with tannic acid and localized on pH 4.3, and 8.0 and 9.5 gels, respectively. After the gels were rinsed in buffer solutions, the activities of the enzymes were recovered in good yield from the gels. The use of these techniques is discussed. © 1979.

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页码：575 / 578

页数：4

相关论文

共 12 条

[1] PURIFICATION AND SOME PROPERTIES OF YEAST TANNASE [J].

AOKI, K ;

SHINKE, R ;

NISHIRA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (01) :79-85

[2] CHEMICAL COMPOSITION AND MOLECULAR-WEIGHT OF YEAST TANNASE [J].

AOKI, K ;

SHINKE, R ;

NISHIRA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (02) :297-302

[3] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[4] A NEW METHOD FOR MICRODETERMINATION OF AMYLASE ACTIVITY BY THE USE OF AMYLOSE AS THE SUBSTRATE [J].

FUWA, H .

JOURNAL OF BIOCHEMISTRY, 1954, 41 (05) :583-603

[5]

GABRIEL O, 1971, METHOD ENZYMOL, V22, P578

[6] A METHOD FOR IMMEDIATE VISUALIZATION OF PROTEINS IN ACRYLAMIDE GELS AND ITS USE FOR PREPARATION OF ANTIBODIES TO ENZYMES [J].

HARTMAN, BK ;

UDENFRIEND, S .

ANALYTICAL BIOCHEMISTRY, 1969, 30 (03) :391-+

[7] MURAMIDASE CATALYZED HYDROLYSIS OF GLYCOL CHITIN [J].

HAYASHI, K ;

FUNATSU, M ;

YAMASAKI, N .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1964, 28 (08) :517-&

[8]

NISHIRA H, 1963, 16 HYOG U AGR MEM, P1

[9] DISK ELECTROPHORESIS OF BASIC PROTEINS AND PEPTIDES ON POLYACRYLAMIDE GELS [J].

REISFELD, RA ;

LEWIS, UJ ;

WILLIAMS, DE .

NATURE, 1962, 195 (4838) :281-&

[10] STRUCTURAL TRANSITIONS OF LYSOZYME [J].

STEINER, RF .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 79 (01) :51-&