DETECTION OF GENITAL PAPILLOMAVIRUS TYPES BY POLYMERASE CHAIN-REACTION USING COMMON PRIMERS

被引：12

作者：

JENKINS, A

KRISTIANSEN, BE

ASK, E

OSKARSEN, B

KRISTIANSEN, E

LINDQVIST, B

TROPE, C

KJORSTAD, K

机构：

[1] UNIV OSLO, INST BIOL, OSLO 3, NORWAY

[2] NORWEGIAN RADIUM HOSP, OSLO 3, NORWAY

来源：

APMIS | 1991年 / 99卷 / 07期

关键词：

HPV; PCR; CERVICAL CANCER;

D O I：

10.1111/j.1699-0463.1991.tb01244.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We describe the detection of eight genital human papillomavirus (HPV) types, including HPV16 and HPV18, by PCR amplification of a 323 base-pair region of the genome within the L1 open reading frame (ORF). The primer sequences are: TGYAAATATCCWGATTWTWT and GTATCWACMACAGTAACAAA. The method will detect purified HPV16 DNA down to a concentration of as little as a single molecule in 100-mu-1. The method is also applicable to purified DNA and crude lysates from tumour biopsies. Typing of the PCR product can be achieved with specific oligonucleotide probes.

引用

收藏

页码：667 / 673

页数：7

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