THE HYDROLYSIS OF TRIGLYCERIDES BY IMMOBILIZED LIPASE IN A HYDROPHILIC MEMBRANE REACTOR

被引：102

作者：

PRONK, W

KERKHOF, PJAM

VANHELDEN, C

VANTRIET, K

机构：

[1] Agricultural Univ Wageningen, Netherlands

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1988年 / 32卷 / 04期

关键词：

Cell Culture--Batch - Enzymes--Immobilization - Lipids--Hydrolysis - Membranes--Liquids;

D O I：

10.1002/bit.260320414

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present article a method is described to immobilize lipase from Candida rugosa on a hollow fiber membrane, and the use of such a system for the hydrolysis of lipids is reported. The membranes were ENKA hydrophilic Cuprophan-type hollow fibers, having a large specific surface area. The immobilized lipase exhibited a high stability: the half-life time was 43 days at a temperature of 30°C. Furthermore, it is proved that kinetic studies can be carried out with this system, operated in a batch of continuous mode. The relation between conversion rate and degree of hydrolysis was determined. On this basis, a dynamic model of the process was developed that describes the relation between reaction conditions and the conversion rate.

引用

页码：512 / 518

页数：7