HUMAN LYMPHOCYTES-T SYNTHESIZE AND SECRETE A PROTEASE RESISTANT PROTEOGLYCAN IN A DELAYED, SERUM-DEPENDENT RESPONSE TO CONCANAVALIN-A

被引：4

作者：

CHOPRA, R ^{[1
]}

GRIFFITH, E ^{[1
]}

IRWIN, D ^{[1
]}

ANASTASSIADES, T ^{[1
]}

机构：

[1] QUEENS UNIV,DEPT BIOCHEM,KINGSTON K7L 3N6,ONTARIO,CANADA

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1991年 / 15卷 / 01期

关键词：

D O I：

10.1016/0309-1651(91)90079-X

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the presence of radioactive precursors, the addition of Concanavalin A (Con A) to cultures of mononuclear cells (MC) from human blood resulted in marked accumulation in the medium of a protease-resistant proteoglycan (PG) with chondroitin sulfate (CS) chains of an average molecular weight of 28 KDa. This PG was shown to be elaborated primarily by T-cells. The addition of serum was necessary for maximal stimulation with Con A and under these conditions PG accumulation in the medium rose slowly during the first few days of culture and much more rapidly between days 6-8. © 1991.

引用

页码：25 / 35

页数：11

共 22 条

[1]

AGRAWAL BBL, 1972, METHOD ENZYMOL, V28, P313

[2] COMPARISON OF THE STIMULATION OF GLYCOSAMINOGLYCAN SYNTHESIS IN CULTURES OF MONONUCLEAR-CELLS FROM BLOOD AND OF FIBROBLASTIC CELLS [J].

ANASTASSIADES, T ;

WOOD, A ;

ELLIOTT, M ;

STEPHENS, C ;

KISILEVSKY, R .

CELL DIFFERENTIATION, 1987, 21 (01) :37-46

[3]

ANASTASSIADES T, 1984, J RHEUMATOL, V11, P729

[4]

ANASTASSIADES T, 1985, MED BIOL, V63, P123

[5] EFFECT OF SOLUBLE PRODUCTS FROM LECTIN-STIMULATED LYMPHOCYTES ON THE GROWTH, ADHESIVENESS, AND GLYCOSAMINOGLYCAN SYNTHESIS OF CULTURED SYNOVIAL FIBROBLASTIC CELLS [J].

ANASTASSIADES, TP ;

WOOD, A .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 68 (03) :792-802

[6]

ANASTASSIADES TP, 1983, J RHEUMATOL, V10, P539

[7]

BERESFORD JN, 1987, J BIOL CHEM, V262, P17164

[8]

CARLSON DM, 1968, J BIOL CHEM, V243, P616

[9]

HANDWERGER BS, 1974, TRANSPLANTATION, V18, P545

[10]

HRONOWSKI L, 1979, ANAL BIOCHEM, V93, P60, DOI 10.1016/S0003-2697(79)80046-9

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