DNA HISTOGRAM DEBRIS THEORY AND COMPENSATION

被引：31

作者：

BAGWELL, CB

MAYO, SW

WHETSTONE, SD

HITCHCOX, SA

BAKER, DR

HERBERT, DJ

WEAVER, DL

JONES, MA

LOVETT, EJ

机构：

[1] VERITY SOFTWARE HOUSE INC,MAINE CYTOMETRY RES INST,PORTLAND,ME 04102

[2] MAINE MED CTR,PORTLAND,ME 04102

来源：

CYTOMETRY | 1991年 / 12卷 / 02期

关键词：

DNA HISTOGRAM ANALYSIS; CELL CYCLE ANALYSIS; DEBRIS SUBTRACTION; DEBRIS COMPENSATION; S-PHASE ANALYSIS; PARAFFIN PROCESSING;

D O I：

10.1002/cyto.990120203

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we describe a theory of DNA histogram debris generation and compensation that can be applied to paraffin-embedded frozen tissue preparations. The theory predicts the distribution of fragments generated from single and multiple random sectioning of three-dimensional ellipsoids representing nuclei. The fragment distribution is assumed to be a major component of the underlying debris in DNA histograms. A comparison of S-phase fractions (SPF) from matched tissue prepared by frozen and formalin-fixed paraffin-embedded DNA methods demonstrates the usefulness of the theory.

引用

页码：107 / 118

页数：12

共 15 条

[11] METHOD FOR ANALYSIS OF CELLULAR DNA CONTENT OF PARAFFIN-EMBEDDED PATHOLOGICAL MATERIAL USING FLOW-CYTOMETRY [J].