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NATIVE HUMAN SERUM AMYLOID P-COMPONENT IS A SINGLE PENTAMER

被引：23
作者：
SORENSEN, IJ
ANDERSEN, O
NIELSEN, EH
SVEHAG, SE
机构：
[1] ODENSE UNIV,DEPT ANAT & CYTOL,DK-5000 ODENSE C,DENMARK
[2] ODENSE UNIV HOSP,DEPT INFECT DIS,DK-5000 ODENSE,DENMARK
来源：
SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1995年 / 41卷 / 03期
关键词：
D O I：
10.1111/j.1365-3083.1995.tb03562.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Serum amyloid P component (SAP) and C-reactive protein (CRP) are members of the pentraxin protein family. SAP is the precursor protein to amyloid P component present in all forms of amyloidosis. The prevailing notion is that SAP in circulation has the form of a double pentameric molecule (decamer) whereas CRP is a single pentameric molecule. We have investigated by gel permeation chromatography the M(r) of SAP in freshly collected human serum and of SAP purified by carbohydrate affinity chromatography and anion exchange chromatography. SAP was monitored by quantitative immunoelectrophoresis and ELISA, and SAP peak fractions were analysed by use of SDS-PAGE, Western blotting, and electron microscopy. The results indicate that native SAP circulates as a single pentamer, a part of which forms complexes with C4b-binding protein. The properties of SAP changed during purification as indicated by rocket immunoelectrophoresis and electron microscopy. Thus, electron micrographs of purified SAP showed a predominance of decamers. However, the decamer form of SAP reversed to single pentamers when purified SAP was incorporated into SAP-depleted serum.
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页码：263 / 267
页数：5
相关论文
共 24 条
[11]   CHARACTERIZATION OF C-REACTIVE PROTEIN AND COMPLEMENT SUBCOMPONENT C1T AS HOMOLOGOUS PROTEINS DISPLAYING CYCLIC PENTAMERIC SYMMETRY (PENTRAXINS) [J].
OSMAND, AP ;
FRIEDENSON, B ;
GEWURZ, H ;
PAINTER, RH ;
HOFMANN, T ;
SHELTON, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :739-743
[12]  
PAINTER RH, 1977, J IMMUNOL, V119, P2203
[13]   THE STRUCTURE AND BINDING CHARACTERISTICS OF SERUM AMYLOID PROTEIN (9.5S ALPHA-1-GLYCOPROTEIN) [J].
PAINTER, RH ;
DEESCALLON, I ;
MASSEY, A ;
PINTERIC, L ;
STERN, SB .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1982, 389 (JUN) :199-215
[14]   ANALOGS IN OTHER MAMMALS AND IN FISH OF HUMAN-PLASMA PROTEINS, C-REACTIVE PROTEIN AND AMYLOID-P COMPONENT [J].
PEPYS, MB ;
DASH, AC ;
FLETCHER, TC ;
RICHARDSON, N ;
MUNN, EA ;
FEINSTEIN, A .
NATURE, 1978, 273 (5658) :168-170
[15]   ACUTE PHASE PROTEINS WITH SPECIAL REFERENCE TO C-REACTIVE PROTEIN AND RELATED PROTEINS (PENTAXINS) AND SERUM AMYLOID A-PROTEIN [J].
PEPYS, MB ;
BALTZ, ML .
ADVANCES IN IMMUNOLOGY, 1983, 34 :141-212
[16]  
PERKINS SJ, 1986, PROTIDES BIOL FLUIDS, V34, P323
[17]  
PINTERIC L, 1976, J IMMUNOL, V117, P79
[18]  
SCHWALBE RA, 1990, J BIOL CHEM, V265, P21749
[19]  
SKINNER M, 1974, J LAB CLIN MED, V84, P604
[20]  
SORENSEN IJ, IN PRESS INT ARCH AL
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