INHIBITION OF NERVE GROWTH FACTOR-INDUCED NEURITE OUTGROWTH OF PC12 CELLS BY A PROTEIN-KINASE INHIBITOR WHICH DOES NOT PERMEATE THE CELL-MEMBRANE

被引：57

作者：

NAGASHIMA, K ^{[1
]}

NAKANISHI, S ^{[1
]}

MATSUDA, Y ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,3-6-6 ASAHIMACHI,MACHIDA,TOKYO 194,JAPAN

来源：

FEBS LETTERS | 1991年 / 293卷 / 1-2期

关键词：

NERVE GROWTH FACTOR; NEURITE OUTGROWTH; PC12; K-252A; K-252B; ECTOPROTEIN KINASE;

D O I：

10.1016/0014-5793(91)81165-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

K-252a, a protein kinase inhibitor of microbial origin, has proven to be a specific inhibitor of nerve growth factor. In this study, the effects of K-252b, the 9-carboxylic acid derivative of K-252a, on nerve growth factor-induced neurite outgrowth in PC12 cells was examined. K-252b is hydrophilic and does not permeate the cell membrane of PC12 cells, whereas K-252a clearly does. K-252b is, however, as potent as K-252a itself in inhibiting the nerve growth factor-induced neurite outgrowth. These results can be interpreted to suggest that effects of K-252b may be through surface-bound/anchored K-252b-sensitive molecules on PC12 cells.

引用

页码：119 / 123

页数：5

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