DIFFERENTIAL-EFFECTS OF THE PROTEIN-KINASE INHIBITOR K-252A ON THE INVITRO SURVIVAL OF CHICK EMBRYONIC NEURONS

被引：54

作者：

BORASIO, GD ^{[1
]}

机构：

[1] MAX PLANCK INST PSYCHIAT,DEPT NEUROCHEM,W-8033 MARTINSRIED,GERMANY

来源：

NEUROSCIENCE LETTERS | 1990年 / 108卷 / 1-2期

关键词：

Ciliary neuronotrophic factor; Embryonic neuron; K-252a; Nerve growth factor; Protein kinase inhibitor; Signal transduction;

D O I：

10.1016/0304-3940(90)90732-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of the protein kinase inhibitor K-252a on the in vitro survival of different populations of chick embryonic neurons were tested. Following dissociation from the corresponding ganglia by trituration, the neurons were cultured on laminin-coated dishes in both the presence and absence of their respective neurotrophic factor. K-252a alone promoted long-term neuronal survival of dorsal root ganglion (DRG) and ciliary ganglion (CG) neurons in a dose-dependent fashion. No such effect was seen with sympathetic ganglion (SG) neurons. In addition, K-252a did not interfere with nerve growth factor (NGF)- or ciliary neuronotrophic factor (CNTF)-mediated survival of DRG or CG neurons, respectively, but completely blocked the NGF- and CNTF-induced survival of SG neurons. High potassium-induced survival of SG neurons was not affected by K-252a. These results point to differences between various neuronal populations in the signal transduction mechanism for neurotrophic factors. © 1990.

引用

页码：207 / 212

页数：6

共 19 条

[1] METHYLTRANSFERASE INHIBITORS BLOCK NGF-REGULATED SURVIVAL AND PROTEIN-PHOSPHORYLATION IN SYMPATHETIC NEURONS [J].

ACHESON, A ;

VOGL, W ;

HUTTNER, WB ;

THOENEN, H .

EMBO JOURNAL, 1986, 5 (11) :2799-2803

[2] PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR [J].

BARBIN, G ;

MANTHORPE, M ;

VARON, S .

JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) :1468-1478

[3] WHAT, IF ANYTHING, IS A NEUROTROPHIC FACTOR [J].

BARDE, YA .

TRENDS IN NEUROSCIENCES, 1988, 11 (08) :343-346

[4] MICROINJECTION OF THE RAS ONCOGENE PROTEIN INTO PC12 CELLS INDUCES MORPHOLOGICAL-DIFFERENTIATION [J].

BARSAGI, D ;

FERAMISCO, JR .

CELL, 1985, 42 (03) :841-848

[5]

BORASIO GD, 1989, NEURON, V2, P1087

[6] AXON INITIATION BY CILIARY NEURONS IN CULTURE [J].

COLLINS, F .

DEVELOPMENTAL BIOLOGY, 1978, 65 (01) :50-57

[7] K-252A SPECIFICALLY INHIBITS THE SURVIVAL AND MORPHOLOGICAL-DIFFERENTIATION OF NGF-DEPENDENT NEURONS IN PRIMARY CULTURES OF HUMAN DORSAL-ROOT GANGLIA [J].

DOHERTY, P ;

WALSH, FS .

NEUROSCIENCE LETTERS, 1989, 96 (01) :1-6

[8] THE HEPARIN-BINDING DOMAIN OF LAMININ IS RESPONSIBLE FOR ITS EFFECTS ON NEURITE OUTGROWTH AND NEURONAL SURVIVAL [J].

EDGAR, D ;

TIMPL, R ;

THOENEN, H .

EMBO JOURNAL, 1984, 3 (07) :1463-1468

[9] SUB-POPULATIONS OF CULTURED CHICK SYMPATHETIC NEURONS DIFFER IN THEIR REQUIREMENTS FOR SURVIVAL FACTORS [J].

EDGAR, D ;

BARDE, YA ;

THOENEN, H .

NATURE, 1981, 289 (5795) :294-295

[10] INHIBITION OF GROWTH FACTOR-INDUCED DIFFERENTIATION OF PC12 CELLS BY MICROINJECTION OF ANTIBODY TO RAS P21 [J].

HAGAG, N ;

HALEGOUA, S ;

VIOLA, M .

NATURE, 1986, 319 (6055) :680-682

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