DIFFERENTIAL-EFFECTS OF THE PROTEIN-KINASE INHIBITOR K-252A ON THE INVITRO SURVIVAL OF CHICK EMBRYONIC NEURONS

被引：54

作者：

BORASIO, GD ^{[1
]}

机构：

[1] MAX PLANCK INST PSYCHIAT,DEPT NEUROCHEM,W-8033 MARTINSRIED,GERMANY

来源：

NEUROSCIENCE LETTERS | 1990年 / 108卷 / 1-2期

关键词：

Ciliary neuronotrophic factor; Embryonic neuron; K-252a; Nerve growth factor; Protein kinase inhibitor; Signal transduction;

D O I：

10.1016/0304-3940(90)90732-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of the protein kinase inhibitor K-252a on the in vitro survival of different populations of chick embryonic neurons were tested. Following dissociation from the corresponding ganglia by trituration, the neurons were cultured on laminin-coated dishes in both the presence and absence of their respective neurotrophic factor. K-252a alone promoted long-term neuronal survival of dorsal root ganglion (DRG) and ciliary ganglion (CG) neurons in a dose-dependent fashion. No such effect was seen with sympathetic ganglion (SG) neurons. In addition, K-252a did not interfere with nerve growth factor (NGF)- or ciliary neuronotrophic factor (CNTF)-mediated survival of DRG or CG neurons, respectively, but completely blocked the NGF- and CNTF-induced survival of SG neurons. High potassium-induced survival of SG neurons was not affected by K-252a. These results point to differences between various neuronal populations in the signal transduction mechanism for neurotrophic factors. © 1990.

引用

页码：207 / 212

页数：6

共 19 条

[11] K-252A, A POTENT PROTEIN-KINASE INHIBITOR, BLOCKS NERVE GROWTH-FACTOR INDUCED NEURITE OUTGROWTH AND CHANGES IN THE PHOSPHORYLATION OF PROTEINS IN PC12H CELLS [J].

HASHIMOTO, S .

JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1531-1539

[12] BRAIN-DERIVED NEUROTROPHIC FACTOR PREVENTS NEURONAL DEATH INVIVO [J].

HOFER, MM ;

BARDE, YA .

NATURE, 1988, 331 (6153) :261-262

[13] CILIARY NEUROTROPHIC FACTOR INDUCES TYPE-2 ASTROCYTE DIFFERENTIATION IN CULTURE [J].

HUGHES, SM ;

LILLIEN, LE ;

RAFF, MC ;

ROHRER, H ;

SENDTNER, M .

NATURE, 1988, 335 (6185) :70-73

[14] K-252A, A POTENT INHIBITOR OF PROTEIN-KINASE-C FROM MICROBIAL ORIGIN [J].

KASE, H ;

IWAHASHI, K ;

MATSUDA, Y .

JOURNAL OF ANTIBIOTICS, 1986, 39 (08) :1059-1065

[15]

KOIZUMI S, 1988, J NEUROSCI, V8, P715

[16] PLACODE AND NEURAL CREST-DERIVED SENSORY NEURONS ARE RESPONSIVE AT EARLY DEVELOPMENTAL STAGES TO BRAIN-DERIVED NEUROTROPHIC FACTOR [J].

LINDSAY, RM ;

THOENEN, H ;

BARDE, YA .

DEVELOPMENTAL BIOLOGY, 1985, 112 (02) :319-328

[17] INHIBITION BY K-252A, A NEW INHIBITOR OF PROTEIN-KINASE, OF NERVE GROWTH FACTOR-INDUCED NEURITE OUTGROWTH OF CHICK-EMBRYO DORSAL-ROOT GANGLION-CELLS [J].

MATSUDA, Y ;

FUKUDA, J .

NEUROSCIENCE LETTERS, 1988, 87 (1-2) :11-17

[18] SARCOMA-VIRUSES CARRYING RAS ONCOGENES INDUCE DIFFERENTIATION-ASSOCIATED PROPERTIES IN A NEURONAL CELL-LINE [J].

NODA, M ;

KO, M ;

OGURA, A ;

LIU, DG ;

AMANO, T ;

TAKANO, T ;

IKAWA, Y .

NATURE, 1985, 318 (6041) :73-75

[19] PHYSIOLOGY OF NERVE GROWTH-FACTOR [J].

THOENEN, H ;

BARDE, YA .

PHYSIOLOGICAL REVIEWS, 1980, 60 (04) :1284-1335

← 1 2 →