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THE MURINE LUTEINIZING-HORMONE AND FOLLICLE-STIMULATING-HORMONE RECEPTOR GENES - TRANSCRIPTION INITIATION SITES, PUTATIVE PROMOTER SEQUENCES AND PROMOTER ACTIVITY

被引：79
作者：
HUHTANIEMI, IT [1 ]
ESKOLA, V [1 ]
PAKARINEN, P [1 ]
MATIKAINEN, T [1 ]
SPRENGEL, R [1 ]
机构：
[1] UNIV HEIDELBERG, ZENTRUM MOLEK BIOL, MOLEC NEUROENDOCRINOL LAB, W-6900 HEIDELBERG 1, GERMANY
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 88卷 / 1-3期
基金：
芬兰科学院;
关键词：
FOLLICLE-STIMULATING HORMONE RECEPTOR; LUTEINIZING HORMONE RECEPTOR; PROMOTER; GENE STRUCTURE; TRANSCRIPTION INITIATION SITE; LUCIFERASE REPORTER GENE;
D O I：
10.1016/0303-7207(92)90009-U
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The putative promoter regions of the murine follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptor genes were isolated and used to map transcription initiation sites for both genes. For the FSH receptor gene, a major transcription initiation site was found 534 nucleotides upstream, and for the LH receptor gene 310 nucleotides upstream of the corresponding translation initiation codons. In addition, several alternative minor transcription initiation sites were observed for both genes. The nucleotide sequences of the promoter regions revealed no canonical promoter elements, such as TATA and CCAAT consensus sites 5' of the main transcriptional start sites. The isolated promoter segments for both receptor genes showed low functional activity as verified in transient expression studies in immature rat granulosa cells using the luciferase coding region as the reporter for promoter activity. Both promoter elements seem to be still under tissue specific control, since neither LH receptor nor FSH receptor promoter activity was detectable in another cell line (CHO) investigated.
引用
收藏
页码：55 / 66
页数：12
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