PCR-BASED IMMORTALIZATION AND SCREENING OF HIERARCHICAL POOLS OF CDNAS

被引：19

作者：

DESPOSITO, M

MAZZARELLA, R

PENGUE, G

JONES, C

DURSO, M

SCHLESSINGER, D

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,SCH MED,CTR MED GENET,ST LOUIS,MO 63110

[3] CNR,INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 22期

关键词：

D O I：

10.1093/nar/22.22.4806

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the relative abundance of the mRNA species. Isolation of a cDNA clone can be done in less than a week. cDNAs were isolated that were cognate for fragments of expressed sequences and for an exon predicted from genomic sequence.

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页码：4806 / 4809

页数：4

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