THE WNT-1 PROTOONCOGENE INDUCES CHANGES IN MORPHOLOGY, GENE-EXPRESSION, AND GROWTH-FACTOR RESPONSIVENESS IN PC12 CELLS

被引：53

作者：

SHACKLEFORD, GM

WILLERT, K

WANG, JW

VARMUS, HE

机构：

[1] UNIV SO CALIF,SCH MED,DEPT PEDIAT,LOS ANGELES,CA 90033

[2] UNIV SO CALIF,SCH MED,DEPT MICROBIOL,LOS ANGELES,CA 90033

[3] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

[4] UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143

来源：

NEURON | 1993年 / 11卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0896-6273(93)90116-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The product of the Wnt-1 proto-oncogene is a secreted glycoprotein that is normally produced in regions of the embryonic neural tube. We show here that expression of mouse Wnt-1 cDNA in the rat PC12 pheochromocytoma cell line causes a dramatic conversion from a round to a flat cell morphology. In addition, PC12 cells expressing Wnt-1 (PC12/Wnt-1) fail to extend neurites after treatment with NGF, despite the presence and activation of high affinity NGF receptors encoded by the trk gene and the induction of early response genes. Furthermore, PC12/Wnt-1 cells fail to express several neuron- and chromaffin-specific genes, indicating that PC12/Wnt-1 cells have assumed a new phenotype. Although NGF and FGF utilize similar signal transduction pathways in PC12 cells, only FGF is capable of inducing a morphological response and synthesis of transin mRNA in PC12/Wnt-1 cells.

引用

页码：865 / 875

页数：11

共 70 条

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