THE GENE FOR CAMPYLOBACTER TRIGGER FACTOR - EVIDENCE FOR MULTIPLE TRANSCRIPTION START SITES AND PROTEIN PRODUCTS

被引：12

作者：

GRIFFITHS, PL

PARK, RWA

CONNERTON, IF

机构：

[1] UNIV READING, DEPT MICROBIOL, READING RG6 2AJ, BERKS, ENGLAND

[2] INST FOOD RES, DEPT PROT ENGN, READING LAB, READING RG6 2EF, BERKS, ENGLAND

来源：

MICROBIOLOGY-SGM | 1995年 / 141卷

基金：

英国惠康基金;

关键词：

CAMPYLOBACTER JEJUNI; TRIGGER FACTOR; TIG; CLPP PROTEASE; GTP CYCLOHYDROLASE;

D O I：

10.1099/13500872-141-6-1359

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A gene encoding a protein of apparent molecular mass 56 kDa that shares 31% identity with the amino acid sequence of trigger factor from Escherichia coli (a protein thought to be involved in cell division), was cloned from Campylobacter jejuni NCTC 11168. The clone was selected from a lambda ZAP tl genomic DNA library following an immune-screen using antiserum raised against glycine-extractable proteins from C. jejuni. The gene has two potential initiation codons. giving rise to two possible nested protein products. Complex differential growth-phase-dependent transcripts give rise to these products.

引用

收藏

页码：1359 / 1367

页数：9

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