IMMOBILIZATION OF MICROBIAL PROTEASE ON VERMICULITE

被引：4

作者：

CHELLAPANDIAN, M ^{[1
]}

SASTRY, CA ^{[1
]}

机构：

[1] INDIAN INST TECHNOL,DEPT CHEM ENGN,MADRAS 600036,TAMIL NADU,INDIA

来源：

BIOPROCESS ENGINEERING | 1992年 / 8卷 / 1-2期

关键词：

D O I：

10.1007/BF00369261

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vermiculite, an inert and cheap solid support material, was used in the immobilization of protease by adsorption. Adsorption of protease on vermiculite saturated with potassium, calcium and aluminium was studied. Aluminium saturated vermiculite adsorbed maximum amount of enzyme at pH 6.5. The maximum adsorption of enzyme on cationic vermiculite occurred within one hour at 30-degrees-C. When the temperature was increased there was a two fold increase in the adsorption of the enzyme. From the Freundlich isotherm data, the values of k and n were computed.

引用

页码：33 / 38

页数：6

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