CLONING AND 5'-FLANKING SEQUENCE OF A RAT CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE

被引：29

作者：

CHIANG, JYL

YANG, TP

WANG, DP

机构：

[1] Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown, OH

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 03期

关键词：

CHOLESTEROL; 7-ALPHA-HYDROXYLASE; GENE CLONING; GENE SEQUENCE; REGULATORY ELEMENT;

D O I：

10.1016/0167-4781(92)90175-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cholesterol 7alpha-hydroxylase gene containing 8 kb of the 5-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1, OCT-1). In addition, 21 CA repeats which have potential to form the Z-DNA structure were found in this region. These putative regulatory elements and repetitive motifs may play roles in the regulation of cholesterol 7alpha-hydroxylase gene in the liver. The sequence identity of the rat gene to the human gene in this region is low. Only liver-enriched elements are conserved in the cholesterol 7alpha-hydroxylase genes.

引用

页码：337 / 339

页数：3

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