PLASMA DEHYDROEPIANDROSTERONE SULFATE SERVES AS A PREHORMONE FOR 48-PERCENT OF FOLLICULAR-FLUID TESTOSTERONE DURING TREATMENT WITH MENOTROPINS

Evidence has suggested that dehydroepiandrosterone sulfate (DS) is a prehormone for ovarian steroidogenesis. To study this hypothesis, combined 6-h infusions of [H-3]dehydroepiandrosterone sulfate and [C-14]testosterone ([C-14]T) were performed in four normal women treated with menotropins who were undergoing medically indicated surgery, and the data were compared to those from nine normal women. The concentrations of tracer and nonisotopic DS, androst-5-ene-3beta,17beta-diol sulfate (DELTA5diolS), androst-5-ene-3beta,17beta-diol (DELTA5diol), dehydroepiandrosterone (D), androstenedione (DELTA4A), and T were determined in arterial and venous blood and in follicular fluid. From these data, the concentrations and fractions of steroids in the follicular fluid that were derived from DS were calculated from the specific activity of intravascular DS and the concentrations of follicular fluid tracer steroids and their specific activities. The fractions of T (0.48), DELTA5diol (0.31), DELTA5diolS (0.42), and D (0.25) in follicular fluid arising from circulating DS were similar and were not significantly different from that of follicular DS arising from circulating DS (0.32). However, the fraction of follicular fluid DELTA4A (0.041) was significantly less (P < 0.01), and the fractions of intrafollicular estrone and estradiol arising from DS were both less than 0.04. The mean MCR of DS in the women treated with menotropins of 22.0 +/- 3.5 L/day (mean +/- SE) was significantly higher than the normal control value. These findings elucidate an important mechanism of adrenal/ovarian interaction at the level of steroidogenesis; circulating DS serves as a prehormone for the production of intrafollicular DELTA5diolS, DELTA5diol, D, and T.