HLA-DPB1 ALLELE MISMATCHES BETWEEN UNRELATED HLA-A,B,C,DR (GENERIC) DQA1-IDENTICAL UNRELATED INDIVIDUALS WITH UNREACTIVE MLC

被引：13

作者：

SALAZAR, M ^{[1
]}

YUNIS, I ^{[1
]}

ALOSCO, SM ^{[1
]}

CHOPEK, M ^{[1
]}

YUNIS, EJ ^{[1
]}

机构：

[1] AMER RED CROSS,DEDHAM,MA

来源：

TISSUE ANTIGENS | 1992年 / 39卷 / 04期

关键词：

HLA-DPB1; MHC; MLC; PCR-RFLP;

D O I：

10.1111/j.1399-0039.1992.tb01936.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have used a PCR-RFLP method with one generic amplification of HLA-DPB1 second exon and 6 endonucleases to differentiate the 19 HLA-DPB1 alleles and 171 heterozygous combinations. The set of primers used in our studies produced fragment sizes different from those published before (1). The HLA-DPB1 alleles in Caucasians showed a higher frequency of DPB1*0401 and DPB1*0402, when compared to a small group of Colombians who showed a higher frequency of DPB1*0402 and DPB1*0201. We found three HLA-DPB1 alleles associated with two HLA haplotypes that result from non-random association of alleles: DPB1*0401 with HLA-A26. B38, DR4, DQA1*0301 and DPB1*0101 and DPB1*0401 with HLA-A1. B8, DR3, DQA1*0501. We also report that 70% of combinations between HLA (generic A,B,C,DR) and DQA1-identical MLC-unreactive cell mixtures showed HLA-DPB1 mismatches. suggesting that HLA-DPB1 differences are not important in MLC reactivity.

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页码：203 / 208

页数：6

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