CHANGES IN PEROXIDASE-ACTIVITY ASSOCIATED WITH CELL-WALLS DURING PINE HYPOCOTYL GROWTH

被引:69
作者
SANCHEZ, M
REVILLA, G
ZARRA, I
机构
[1] Laboratorio de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago
关键词
CELL WALL; GROWTH; HYPOCOTYL; PEROXIDASE; PINE; PINUS PINASTER AITON;
D O I
10.1006/anbo.1995.1039
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Peroxidases active against 2,2'-azino-bis-[3-ethylbenzthiazoline-6-acid] (ABTS) and guaiacol were found in the apoplastic fluid, as well as ionically and covalently associated with pine cell walls. The highest activity was found covalently bound to cell walls, while the lowest activity was in the apoplastic fluid. Both ABTS and guaiacol peroxidases increased with the hypocotyl age in the three fractions, apoplastic, ionically and covalently bound. Furthermore, the changes in both peroxidases along the hypocotyl were also studied. Both apoplastic ABTS- and guaiacol-peroxidases increased from the apical towards the basal region of the hypocotyls of 10-d-old seedlings. A relation between peroxidase activity in the apoplastic fluid and the cell wall stiffening in pine hypocotyls is proposed.
引用
收藏
页码:415 / 419
页数:5
相关论文
共 21 条
[11]   CELL-WALL AUTOLYSIS IN PINUS-PINASTER AITON HYPOCOTYLS ENZYMATIC-ACTIVITIES INVOLVED [J].
LLAMAZARES, J ;
ACEBES, JL ;
ZARRA, I .
JOURNAL OF PLANT PHYSIOLOGY, 1987, 127 (1-2) :11-22
[12]  
LORENCES EP, 1990, PHYSIOL PLANTARUM, V79, P563, DOI 10.1111/j.1399-3054.1990.tb02118.x
[13]   HYPOCOTYL GROWTH OF PINUS-PINASTER SEEDLINGS - CHANGES IN OSMOTIC POTENTIAL AND CELL-WALL COMPOSITION [J].
LORENCES, EP ;
ZARRA, I .
PHYSIOLOGIA PLANTARUM, 1986, 67 (03) :377-382
[14]  
Putter J., 1974, METHODS ENZYMATIC AN, P685, DOI [10.1016/B978-0-12-091302-2.50033-5, DOI 10.1016/B978-0-12-091302-2.50033-5]
[15]   LUPIN PEROXIDASES .2. BINDING OF ACIDIC ISOPEROXIDASES TO CELL-WALLS [J].
BARCELO, AR ;
PEDRENO, MA ;
MUNOZ, R ;
SABATER, F .
PHYSIOLOGIA PLANTARUM, 1988, 73 (02) :238-244
[16]   PEROXIDASE REGULATION BY 3-DWARF HEIGHT LOCUS IN SORGHUM [J].
SCHERTZ, KF ;
SUMPTER, NA ;
SARKISSIAN, IV ;
HART, GE .
JOURNAL OF HEREDITY, 1971, 62 (04) :235-+
[17]   EFFECT OF IAA ON GROWTH AND SOLUBLE CELL-WALL POLYSACCHARIDES CENTRIFUGED FROM PINE HYPOCOTYL SECTIONS [J].
TERRY, ME ;
MCGRAW, D ;
JONES, RL .
PLANT PHYSIOLOGY, 1982, 69 (02) :323-326
[18]   VARIATIONS OF CELL-WALL PEROXIDASES IN EPICOTYLS OF CICER-ARIETINUM DURING GROWTH [J].
VALERO, P ;
NICOLAS, G ;
LABRADOR, E .
PLANT SCIENCE, 1991, 74 (02) :171-178
[19]   SOME MOLECULAR ASPECTS OF PLANT PEROXIDASE BIOSYNTHETIC-STUDIES [J].
VANHUYSTEE, RB .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1987, 38 :205-219
[20]  
WHITMORE FW, 1976, PHYTOCHEMISTRY, V15, P375, DOI 10.1016/S0031-9422(00)86826-0