CHARACTERIZATION OF THE GENE ENCODING SUPEROXIDE-DISMUTASE OF BORDETELLA-PERTUSSIS AND CONSTRUCTION OF A SOD-DEFICIENT MUTANT

被引：16

作者：

DESHAZER, D ^{[1
]}

BANNAN, JD ^{[1
]}

MORAN, MJ ^{[1
]}

FRIEDMAN, RL ^{[1
]}

机构：

[1] UNIV ARIZONA, DEPT MICROBIOL & IMMUNOL, TUCSON, AZ 85724 USA

来源：

GENE | 1994年 / 142卷 / 01期

关键词：

OXIDATIVE STRESS; METALLOENZYME; INSERTION MUTAGENESIS; SUICIDE VECTOR; HOMOLOGOUS RECOMBINATION; HUMAN PATHOGEN;

D O I：

10.1016/0378-1119(94)90359-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Bordetella pertussis gene sodB, encoding superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sonAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pel tussis extracts have a single SOD containing Fe3+ as a prosthetic group. A SOD-deficient mutant was obtained by insertional inactivation of sodB in B. pel tussis, confirming that there is only one SOD in this organism.

引用

页码：85 / 89

页数：5

共 32 条

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