REPLACEMENT OF RNA HAIRPINS BY INVITRO SELECTED TETRANUCLEOTIDES

被引：22

作者：

DICHTL, B ^{[1
]}

PAN, T ^{[1
]}

DIRENZO, AB ^{[1
]}

UHLENBECK, OC ^{[1
]}

机构：

[1] UNIV COLORADO, DEPT CHEM & BIOCHEM, BOULDER, CO 80309 USA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 03期

关键词：

D O I：

10.1093/nar/21.3.531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An in vitro selection method based on the autolytic cleavage of yeast t(RNA)Phe by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2 + and the presence of a specific UV-induced crosslink, certain tetranucleotide sequences allow proper folding of the rest of the tRNA molecule, wheras others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNA(Phe) and the anticodon hairpin of E.coli tRNA(Phe) without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally non-essential hairpins in any RNA.

引用

页码：531 / 535

页数：5

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