NEUTRAL ENDOPEPTIDASE CAN HYDROLYZE BETA-AMYLOID(1-40) BUT SHOWS NO EFFECT ON BETA-AMYLOID PRECURSOR PROTEIN-METABOLISM

被引：176

作者：

HOWELL, S

NALBANTOGLU, J

CRINE, P

机构：

[1] UNIV MONTREAL,FAC MED,DEPT BIOCHIM,MONTREAL,PQ H3C 3J7,CANADA

[2] MCGILL UNIV,MONTREAL NEUROL INST,DEPT NEUROL & NEUROSURG,MONTREAL,PQ H3A 2B4,CANADA

来源：

PEPTIDES | 1995年 / 16卷 / 04期

基金：

英国医学研究理事会;

关键词：

BETA-AMYLOID; AMYLOID PRECURSOR PROTEIN; NEUTRAL ENDOPEPTIDASE (EC 3.4.24.11); ALZHEIMERS DISEASE; NEURO2A CELLS;

D O I：

10.1016/0196-9781(95)00021-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High performance liquid chromatographic analyses of incubations of beta-amyloid(1-40) with neutral endopeptidase revealed at least nine product peaks, indicating that neutral endopeptidase can cleave P-amyloid at multiple sites. Mass spectroscopic analysis of hydrolyzed P-amyloid identified at least five cleavage sites, between residues Glu(3)-Phe(4), Gly(9)-Trp(10), Phe(19)-Phe(20), Ala(30)-Ile(31), and Gly(33)-Leu(34). In contrast, amyloid precursor protein metabolism in Neuro2A cells was unaffected by the expression of recombinant neutral endopeptidase in the same cells or by the addition of a secreted form of neutral endopeptidase to spent Neuro2A cell media.

引用

页码：647 / 652

页数：6

共 30 条

[1] ROLE OF THE BETA-AMYLOID PRECURSOR PROTEIN IN ALZHEIMERS-DISEASE
ASHALL, F
GOATE, AM
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (01) : 42 - 46
[2] THE USE OF A MONOCLONAL-ANTIBODY FOR THE RAPID PURIFICATION OF KIDNEY NEUTRAL ENDOPEPTIDASE (ENKEPHALINASE) SOLUBILIZED IN OCTYL GLUCOSIDE
AUBRY, M
BERTELOOT, A
BEAUMONT, A
ROQUES, BP
CRINE, P
[J]. BIOCHEMISTRY AND CELL BIOLOGY, 1987, 65 (04) : 398 - 404
[3] RELEASE OF EXCESS AMYLOID BETA-PROTEIN FROM A MUTANT AMYLOID BETA-PROTEIN PRECURSOR
CAI, XD
GOLDE, TE
YOUNKIN, SG
[J]. SCIENCE, 1993, 259 (5094) : 514 - 516
[4] THE PRODUCTION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY SPECIFIC FOR THE 94,000 DALTON ENKEPHALIN-DEGRADING PEPTIDASE FROM RABBIT KIDNEY BRUSH-BORDER
CRINE, P
LEGRIMELLEC, C
LEMIEUX, E
LABONTE, L
FORTIN, S
BLACHIER, A
AUBRY, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 131 (01) : 255 - 261
[5] ALPHA-2-MACROGLOBULIN AND OTHER PROTEINASE-INHIBITORS DO NOT INTERFERE WITH THE SECRETION OF AMYLOID PRECURSOR PROTEIN IN MOUSE NEUROBLASTOMA-CELLS
DESTROOPER, B
VANLEUVEN, F
VANDENBERGHE, H
[J]. FEBS LETTERS, 1992, 308 (01) : 50 - 53
[6] CLEAVAGE OF AMYLOID-BETA PEPTIDE DURING CONSTITUTIVE PROCESSING OF ITS PRECURSOR
ESCH, FS
KEIM, PS
BEATTIE, EC
BLACHER, RW
CULWELL, AR
OLTERSDORF, T
MCCLURE, D
WARD, PJ
[J]. SCIENCE, 1990, 248 (4959) : 1122 - 1124
[7] SECRETION OF A FUNCTIONAL SOLUBLE FORM OF NEUTRAL ENDOPEPTIDASE-24.11 FROM A BACULOVIRUS-INFECTED INSECT CELL-LINE
FOSSIEZ, F
LEMAY, G
LABONTE, N
PARMENTIERLESAGE, F
BOILEAU, G
CRINE, P
[J]. BIOCHEMICAL JOURNAL, 1992, 284 : 53 - 59
[8] CELLULAR PROCESSING OF BETA-AMYLOID PRECURSOR PROTEIN AND THE GENESIS OF AMYLOID BETA-PEPTIDE
HAASS, C
SELKOE, DJ
[J]. CELL, 1993, 75 (06) : 1039 - 1042
[9] AMYLOID BETA-PEPTIDE IS PRODUCED BY CULTURED-CELLS DURING NORMAL METABOLISM
HAASS, C
SCHLOSSMACHER, MG
HUNG, AY
VIGOPELFREY, C
MELLON, A
OSTASZEWSKI, BL
LIEBERBURG, I
KOO, EH
SCHENK, D
TEPLOW, DB
SELKOE, DJ
[J]. NATURE, 1992, 359 (6393) : 322 - 325
[10] MEMBRANE PEPTIDASES ON HUMAN OSTEOBLAST-LIKE CELLS IN CULTURE - HYDROLYSIS OF CALCITONIN AND HORMONAL-REGULATION OF ENDOPEPTIDASE-24.11
HOWELL, S
CASWELL, AM
KENNY, AJ
TURNER, AJ
[J]. BIOCHEMICAL JOURNAL, 1993, 290 : 159 - 164

← 1 2 3 →