USE OF A RECOMBINANT ANTIGEN IN AN INDIRECT ELISA FOR DETECTING BOVINE ANTIBODY TO CAPRIPOXVIRUS

被引：42

作者：

CARN, VM ^{[1
]}

KITCHING, RP ^{[1
]}

HAMMOND, JM ^{[1
]}

CHAND, P ^{[1
]}

机构：

[1] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,SURREY,ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 49卷 / 03期

关键词：

CAPRIPOXVIRUS; RECOMBINANT ANTIGEN; ELISA; ANTIBODY; BOVINE;

D O I：

10.1016/0166-0934(94)90143-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for the capripoxvirus structural protein P32 was cloned, expressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and purified on glutathione Sepharose. An indirect enzyme linked immunosorbent assay (ELISA) using this antigen was developed to screen bovine sera for antibodies to capripoxvirus. Sequential serum samples from experimentally infected animals tested by ELISA and by virus neutralisation test (VNT) showed that the ELISA was more sensitive and detected antibodies to capripoxvirus earlier post-infection than the VNT.

引用

页码：285 / 294

页数：10

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