MOLECULAR AND GENETIC-CHARACTERIZATION OF THE RHIZOPINE CATABOLISM (MOCABRC) GENES OF RHIZOBIUM-MELILOTI L5-30

被引:58
作者
ROSSBACH, S
KULPA, DA
ROSSBACH, U
DEBRUIJN, FJ
机构
[1] MICHIGAN STATE UNIV, MSU DOE PLANT RES LAB, E LANSING, MI 48824 USA
[2] MICHIGAN STATE UNIV, NSF, CTR MICROBIAL ECOL, E LANSING, MI 48824 USA
[3] MICHIGAN STATE UNIV, DEPT MICROBIOL, E LANSING, MI 48824 USA
来源
MOLECULAR AND GENERAL GENETICS | 1994年 / 245卷 / 01期
关键词
RHIZOSPHERE; NUTRITIONAL MEDIATOR; PERIPLASMIC BINDING PROTEIN; GNTR-LIKE REGULATOR; DEHYDROGENASE;
D O I
10.1007/BF00279746
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rhizopine (L-3-O-methyl-scyllo-inosamine, 3-O-MSI) is a symbiosis-specific compound, which is synthesized in nitrogen-fixing nodules of Medicago sativa induced by Rhizobium meliloti strain L5-30. 3-O-MSI is thought to function as an unusual growth substrate for R. meliloti L5-30, which carries a locus (mos) responsible for its synthesis closely linked to a locus (moc) responsible for its degradation. Here, the essential moc genes were delimited by Tn5 mutagenesis and shown to be organized into two regions, separated by 3 kb of DNA. The DNA sequence of a 9-kb fragment spanning the two moc regions was determined, and four genes were identified that play an essential role in rhizopine catabolism (mocABC and mocR). The analysis of the DNA sequence and the amino acid sequence of the deduced protein products revealed that MocA resembles NADH-dependent dehydrogenases. MocB exhibits characteristic features of periplasmic-binding proteins that are components of high-affinity transport systems. MocC does not share significant homology with any protein in the database. MocR shows homology with the GntR class of bacterial regulator proteins. These results suggest that the mocABC genes are involved in the uptake and subsequent degradation of rhizopine, whereas mocR is likely to play a regulatory role.
引用
收藏
页码:11 / 24
页数:14
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