PROLIFERATION OF RAT PNEUMOCYSTIS-CARINII ON CELLS SHEETED ON MICROCARRIER BEADS IN SPINNER FLASKS

被引：39

作者：

LEE, CH ^{[1
]}

BAUER, NL ^{[1
]}

SHAW, MM ^{[1
]}

DURKIN, MM ^{[1
]}

BARTLETT, MS ^{[1
]}

QUEENER, SF ^{[1
]}

SMITH, JW ^{[1
]}

机构：

[1] INDIANA UNIV,SCH MED,DEPT PHARMACOL & TOXICOL,INDIANAPOLIS,IN 46202

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 06期

关键词：

D O I：

10.1128/JCM.31.6.1659-1662.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

引用

页码：1659 / 1662

页数：4

共 15 条

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