ARRANGEMENT OF PROTEINS O-8 AND O-9 IN OUTER-MEMBRANE OF ESCHERICHIA-COLI K-12 - EXISTENCE OF HOMOTRIMERS AND HETEROTRIMERS

被引：29

作者：

ICHIHARA, S

MIZUSHIMA, S

机构：

[1] Laboratory of Microbiology, Faculty of Agriculture, Nagoya University, Nagoya-Shi, Aichi-Ken, 464, Chikusa-ku

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 100卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb04174.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular arrangement of major outer membrane proteins O‐8 and O‐9 that exist as trimers has been studied by means of cross‐linking with dimethylsuberimidate. The cross‐linked samples were examined on a urea/sodium dodecyl sulfate/polyacrylamide gel which was developed to separate cross‐linked trimer and dimer of O‐8 from those of O‐9. Cells simultaneously synthesizing both O‐8 and O‐9 formed heterotrimers (trimers containing both proteins) as well as homotrimers. Quantitative analyses revealed that there was no discrimination between O‐8 and O‐9 in the assembly process to form trimers. When cells were grown sequentially under two different sets of conditions so that the cells synthesized either one of the two proteins in the first stage and the other in the second stage of growth, no heterotrimers were formed. This result indicates that subunit exchange did not take place between trimers which had been incorporated into the outer membrane. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：321 / 328

页数：8

共 34 条

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