LOCALIZATION OF PROANTHOCYANIDINS USING INSITU-HYDROLYSIS WITH SULFURIC-ACID

被引：22

作者：

GUTMANN, M

机构：

[1] Institute of Fruit Culture, University of Munich, W-8050, Freising-Weihenstephan

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 03期

关键词：

PROANTHOCYANIDINS; TANNINS; LOCALIZATION; POLYMERIZATION;

D O I：

10.3109/10520299309104689

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A valuable method for specific localization of proanthocyanidins in plant tissues is given. The specificity is based on the acid hydrolysis of condensed tannins in situ via hot sulfuric acid, yielding bright red anthocyanidins. This treatment did not cause noticeable damage of tissue embedded in glycol methacrylate prior to sectioning. Further proof of specificity was achieved by control staining with the flavan-3-ol-selective p-dimethylaminocinnamaldehyde (DMACA) reagent. In addition, comparison of adjacent sections stained with the DMACA reagent may give coarse information about the degree of polymerization of the proanthocyanidins.

引用

页码：161 / 165

页数：5

共 19 条

[1]

AMELUNXEN F, 1969, CYTOBIOLOGIE, V1, P58

[2] LEUCO-ANTHOCYANINS .1. DETECTION AND IDENTIFICATION OF ANTHOCYANIDINS FORMED FROM LEUCO-ANTHOCYANINS IN PLANT TISSUES [J].