ENHANCED SENSITIVITY IN PCR DETECTION OF LISTERIA-MONOCYTOGENES IN SOFT CHEESE THROUGH USE OF AN AQUEOUS 2-PHASE SYSTEM AS A SAMPLE PREPARATION METHOD

被引：67

作者：

LANTZ, PG

TJERNELD, F

BORCH, E

HAHNHAGERDAL, B

RADSTROM, P

机构：

[1] LUND UNIV,LUND INST TECHNOL,DEPT APPL MICROBIOL,S-22100 LUND,SWEDEN

[2] LUND UNIV,DEPT BIOCHEM,S-22100 LUND,SWEDEN

[3] SWEDISH MEAT RES INST,S-24424 KAVLINGE,SWEDEN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 09期

关键词：

D O I：

10.1128/AEM.60.9.3416-3418.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sample treatment method based on an aqueous two-phase system containing polyethylene glycol and dextran was developed for enhancing sensitivity in the detection of Listeria monocytogenes in soft cheese with PCR. The results suggest that the improved detection sensitivity following partitioning of the cheese homogenate in an aqueous two-phase system may be due to partitioning of the PCR inhibitors to the polyethylene glycol phase.

引用

页码：3416 / 3418

页数：3

共 14 条

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