NMR AND CRYSTALLOGRAPHY - COMPLEMENTARY APPROACHES TO STRUCTURE DETERMINATION

被引：28

作者：

MACARTHUR, MW ^{[1
]}

DRISCOLL, PC ^{[1
]}

THORNTON, JM ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

TRENDS IN BIOTECHNOLOGY | 1994年 / 12卷 / 05期

关键词：

D O I：

10.1016/0167-7799(94)90074-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A knowledge of the three-dimensional structure of a protein is essential to understand how a protein performs its functions. It is also a prime requirement for the rational design of novel sequences with specific structural, chemical or catalytic properties. Until recently, such information could only be obtained from X-ray diffraction studies on protein crystals. In the past few years, however, nuclear magnetic resonance (NMR) spectroscopy in solution has rapidly become established as an effective alternative method. This review briefly examines the two techniques and their relevance to protein engineering and design.

引用

页码：149 / 153

页数：5

共 14 条

[1] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].