FUNCTIONAL-ANALYSIS OF THE BACILLUS-SUBTILIS BACTERIOPHAGE-SPP1 PAC SITE

被引：41

作者：

BRAVO, A ^{[1
]}

ALONSO, JC ^{[1
]}

TRAUTNER, TA ^{[1
]}

机构：

[1] MAX PLANCK INST MOLEC GENET,IHNESTR 73,W-1000 BERLIN 33,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 10期

关键词：

D O I：

10.1093/nar/18.10.2881

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Encapsidation of the DNA of the virulent Bacillus subtilis phage SPP1 follows a processive unidirectional headful-mechanism and initiates at a unique genomic location {pac). We have cloned a fragment of SPP1 DNA containing the pac site flanked by reporter genes Into the chromosome of B. subtilis. Infection of such cells with SPP1 led to highly efficient packaging, initiated at the inserted pac site, of chromosomal DNA. The directionality in the packaging of this DNA was the same as observed with vegetative phage DNA. Mutagenizing the chromosomal pac insert defined an 83 base pair segment containing the pac cleavage site which is sufficient to direct phage specific DNA encapsidation. The packaging recognition signal as defined can also be utilized by the SPP1 related phages 41c, SF6 and @15. © 1990 Oxford University Press.

引用

页码：2881 / 2886

页数：6