NM23-H1 EXPRESSION AND DISEASE RECURRENCE AFTER SURGICAL RESECTION OF SMALL HEPATOCELLULAR-CARCINOMA

被引:34
作者
BOIX, L
BRUIX, J
CAMPO, E
SOLE, M
CASTELLS, A
FUSTER, J
RIVERA, F
CARDESA, A
RODES, J
机构
[1] UNIV BARCELONA,HOSP CLIN & PROV BARCELONA,LIVER UNIT,E-08036 BARCELONA,SPAIN
[2] UNIV BARCELONA,HOSP CLIN & PROV BARCELONA,DEPT PATHOL ANAT,BARCELONA,SPAIN
[3] UNIV BARCELONA,HOSP CLIN & PROV BARCELONA,DEPT SURG,BARCELONA,SPAIN
[4] UNIV BARCELONA,HOSP CLIN & PROV BARCELONA,HORMONAL LAB,BARCELONA,SPAIN
关键词
D O I
10.1016/0016-5085(94)90175-9
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: The nm23-H1 gene is thought to act as a metastasis-suppressor gene. This study investigates the relationship between nm23-H1 messenger RNA (mRNA) expression and intrahepatic tumor recurrence after surgical resection of small hepatocellular carcinoma. Methods: Seventeen cirrhotic patients with solitary hepatocellular carcinoma <5 cm underwent surgical resection. In 7 patients, the neoplasm recurred after a 12-month median follow-up, whereas the other 10 patients were free of disease after a 30-month median follow-up. Both groups were similar according to age, sex, etiology, status of the underlying liver, tumor size, and other pathological characteristics of the neoplasm. nm23-H1 mRNA levels were assessed in matched tumor and surrounding cirrhotic liver samples by Northern blot hybridization using a 900-base pair probe, which is a BamHI fragment of pnm23-H1 recombinant complementary DNA clone encoding the nm23-H1 human gene. Results: Eight of the 10 patients without disease recurrence during follow-up showed nm23-H1 overexpression with an increase ranging between three- and 45-fold when compared with the nontumoral surrounding liver. Only 1 of the 7 patients with tumor recurrence showed higher nm23-H1 mRNA levels in the tumor than in the nonneoplastic sample (P = 0.013). Conclusions: nm23 mRNA overexpression in small solitary hepatocellular carcinoma is associated with a lower recurrence rate after surgical resection, suggesting that this gene may participate in the metastatic dissemination of this neoplasm. © 1994.
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页码:486 / 491
页数:6
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