EFFECTS OF DIADENOSINE POLYPHOSPHATES, ATP AND ANGIOTENSIN-II ON MEMBRANE VOLTAGE AND MEMBRANE CONDUCTANCES OF RAT MESANGIAL CELLS

被引：17

作者：

KLETA, R

HIRSCH, J

HEINDENREICH, S

SCHLUTER, H

ZIDEK, W

SCHLATTER, E

机构：

[1] Medizinische Poliklinik D, Experimentelle Nephrologie, Westfälische Wilhelms-Universität Münster, Münster, D-48149

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1995年 / 430卷 / 05期

关键词：

FAST WHOLE-CELL; PATCH-CLAMP K+ CONDUCTANCE; NONSELECTIVE CATION CONDUCTANCE; CL-; CONDUCTANCE; SHR; WKY; ADENOSINE;

D O I：

10.1007/BF00386166

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Diadenosine polyphosphates have been shown to influence renal perfusion pressure. As mesangial cells may contribute to these effects we investigated the effects of diadenosine triphosphate (Ap(3)A), diadenosine tetraphosphate (Ap(4)A), diadenosine pentaphosphate (Ap(5)A) and diadenosine hexaphosphate (Ap(6)A) on membrane voltage (V-m) and membrane conductance (gm) in mesangial cells (MC) of normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats in primary and long-term culture. We applied the patch-clamp technique in the fast-whole-cell configuration to measure V-m and g(m). To compare the effects of diadenosine polyphosphates with hitherto known agonists we also tested adenosine 5'-triphosphate (ATP) and angiotensin II (Ang II). As there was no significant difference in the V-m values in MC of WKY (-42 +/- 1 mV, n = 70) and SHR rats (-45 +/- 2 mV, n = 99) as well as in the agonist-induced changes of V-m, all data were pooled. The V-m of all the cells was -44 +/- 1 mV (n = 169) and g(m) was 15.9 +/- 1.8 nS (n = 141). Ion-exchange experiments showed the presence of a K+ and a non-selective cation conductance in resting MC whereas a Cl- conductance or a Na+-selective conductance could not be observed. Ap(3)A, Ap(4)A, Ap(5)A, AP(6)A and ATP each at a concentration of 5 mu mol/l, led to a significant depolarization of V-m by 5 +/- 2 mV (n = 14), 7 +/- 1 mV (n = 25), 3 + 1 mV (n = 23), 2 + 1 mV (n = 16), and 14 +/- 2 mV (n = 23), respectively. For Ap(4)A, the most potent diadenosine polyphosphate, we determined the half-maximally effective concentration (EC(50)) as 6 mu mol/l (n = 5-25), for ATP as 2 mu mol/l (n = 9-37), and for Ang II as 8 nmol/l (n = 6-18). Ap(4)A 100 mu mol/l increased g(m) significantly by 55 +/- 20% (n = 16), 100 mu mol/l ATP by 135 +/- 60% (n = 18). The diadenosine polyphosphates examined were able to depolarize V-m (Ang II > ATP > Ap(4)A > Ap(3)A > Ap(5)A > Ap(6)A) by activation of a Cl- conductance and a non-selective cation conductance, as do ATP or Ang II.

引用

页码：713 / 720

页数：8

共 38 条

[1]

Andersson M., Diadenosine tetraphosphate (Ap4A): its presence and functions in biological systems, Int J Biochem, 21, pp. 707-714, (1989)

[2]

Ausiello D.A., Kreisberg J.I., Roy C., Karnovsky M.J., Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin, J Clin Invest, 65, pp. 754-760, (1980)

[3]

Blantz R.C., Konnen K.S., Tucker B.J., Angiotensin II effects upon the glomerular microcirculation and ultrafiltration coefficient of the rat, J Clin Invest, 57, pp. 419-434, (1976)

[4]

Busse R., Ogilvie A., Pohl U., Vasomotor activity of diadenosine triphosphates and diadenosine tetraphosphates in isolated arteries, Am J Physiol, 254, pp. H828-H832, (1988)

[5]

Busshardt E., Gerok W., Haussinger D., Regulation of hepatic parenchymal and non-parenchymal cell function by the diadenine nucleotides Ap3A and Ap4A, Biochim Biophys Acta, 1010, pp. 151-159, (1989)

[6]

Garrison P.N., Barnes L.D., Determination of dinucleoside polyphosphates, Ap<inf>4</inf>A and other dinucleoside polyphosphates, pp. 29-61, (1992)

[7]

Gloy J., Greger R., Schollmeyer P., Huber M., Pavenstadt H., Influence of cell culture conditions and passage number on the response of membrane voltage to ATP and angiotensin II in rat mesangial cells, Renal Physiol Biochem, 17, pp. 62-72, (1994)

[8]

Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J., Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches, Pflügers Arch, 391, pp. 85-100, (1981)

[9]

Harper P.A., Robinson J.M., Hoover R.L., Wright T.C., Karnovsky M.J., Improved methods for culturing rat glomerular cells, Kidney Int, 26, pp. 875-880, (1984)

[10]

Heidenreich S., Tepel M., Lang D., Rahn K-H, Zidek W., Differential effects of insulin-like growth factor I and plateletderived growth factor on growth response, matrix formation, and cytosolic free calcium of glomerular mesangial cells of spontaneously hypertensive and normotensive rats, Nephron, 68, pp. 481-488, (1994)

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