EFFECTS OF DIADENOSINE POLYPHOSPHATES, ATP AND ANGIOTENSIN-II ON MEMBRANE VOLTAGE AND MEMBRANE CONDUCTANCES OF RAT MESANGIAL CELLS

被引：17

作者：

KLETA, R

HIRSCH, J

HEINDENREICH, S

SCHLUTER, H

ZIDEK, W

SCHLATTER, E

机构：

[1] Medizinische Poliklinik D, Experimentelle Nephrologie, Westfälische Wilhelms-Universität Münster, Münster, D-48149

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1995年 / 430卷 / 05期

关键词：

FAST WHOLE-CELL; PATCH-CLAMP K+ CONDUCTANCE; NONSELECTIVE CATION CONDUCTANCE; CL-; CONDUCTANCE; SHR; WKY; ADENOSINE;

D O I：

10.1007/BF00386166

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Diadenosine polyphosphates have been shown to influence renal perfusion pressure. As mesangial cells may contribute to these effects we investigated the effects of diadenosine triphosphate (Ap(3)A), diadenosine tetraphosphate (Ap(4)A), diadenosine pentaphosphate (Ap(5)A) and diadenosine hexaphosphate (Ap(6)A) on membrane voltage (V-m) and membrane conductance (gm) in mesangial cells (MC) of normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats in primary and long-term culture. We applied the patch-clamp technique in the fast-whole-cell configuration to measure V-m and g(m). To compare the effects of diadenosine polyphosphates with hitherto known agonists we also tested adenosine 5'-triphosphate (ATP) and angiotensin II (Ang II). As there was no significant difference in the V-m values in MC of WKY (-42 +/- 1 mV, n = 70) and SHR rats (-45 +/- 2 mV, n = 99) as well as in the agonist-induced changes of V-m, all data were pooled. The V-m of all the cells was -44 +/- 1 mV (n = 169) and g(m) was 15.9 +/- 1.8 nS (n = 141). Ion-exchange experiments showed the presence of a K+ and a non-selective cation conductance in resting MC whereas a Cl- conductance or a Na+-selective conductance could not be observed. Ap(3)A, Ap(4)A, Ap(5)A, AP(6)A and ATP each at a concentration of 5 mu mol/l, led to a significant depolarization of V-m by 5 +/- 2 mV (n = 14), 7 +/- 1 mV (n = 25), 3 + 1 mV (n = 23), 2 + 1 mV (n = 16), and 14 +/- 2 mV (n = 23), respectively. For Ap(4)A, the most potent diadenosine polyphosphate, we determined the half-maximally effective concentration (EC(50)) as 6 mu mol/l (n = 5-25), for ATP as 2 mu mol/l (n = 9-37), and for Ang II as 8 nmol/l (n = 6-18). Ap(4)A 100 mu mol/l increased g(m) significantly by 55 +/- 20% (n = 16), 100 mu mol/l ATP by 135 +/- 60% (n = 18). The diadenosine polyphosphates examined were able to depolarize V-m (Ang II > ATP > Ap(4)A > Ap(3)A > Ap(5)A > Ap(6)A) by activation of a Cl- conductance and a non-selective cation conductance, as do ATP or Ang II.

引用

页码：713 / 720

页数：8

共 38 条

[21]

Mene P., Simonson, Dunn M.J., Physiology of the mesangial cell, Physiol Rev, 69, pp. 1347-1423, (1989)

[22]

Nees S., Coronary flow increases induced by adenosine and adenine nucleotides are mediated by the coronary endothelium: a new principle of the regulation of coronary flow, Eur Heart, 10, pp. 28-35, (1989)

[23]

Ogilvie A., Schulze-Lohoff E., Sterzel R.B., Mesangiumzellen (MZ) in Kultur exprimieren Oberflächenenzyme, die extrazelluläre Nukleotide (ATP, AMP) und Dinukleotide (Ap4A, Ap3A) abbauen (abstract), Nieren Hochdruck, 19, (1990)

[24]

Okuda T., Yamashita N., Kurokawa K., Angiotensin II and vasopressin stimulate calcium-activated chloride conductance in rat mesangial cells, J Clin Invest, 78, pp. 1443-1448, (1986)

[25]

Ortiz B., Sillero A., Gunther, Specific synthesis of adenosine(5′)tetraphospho(5′)nucleoside and adenosine(5′)oligophospho(5′)-adenosine (n>4) catalysed by firefly luciferase, Eur J Biochem, 212, pp. 263-270, (1993)

[26]

Pavenstadt H., Gloy J., Leipziger J., Klar B., Pfeilschifter J., Schollmeyer P., Greger R., Effect of extracellular ATP on contraction, cytosolic calcium activity, membrane voltage and ion currents of rat mesangial cells in primary culture, British Journal of Pharmacology, 109, pp. 953-959, (1993)

[27]

Pavenstadt H., Ruh J., Greger R., Schollmeyer P., Adenosine-induced hyperpolarization of the membrane voltage in rat mesangial cells in primary culture, British Journal of Pharmacology, 113, pp. 7-12, (1994)

[28]

Pintor J., Rotllan P., Torres M., Miras-Portugal M.T., Characterization and quantification of diadenosine hexaphosphate in chromaffin cells: granular storage and secretagogue-induced release, Anal Biochem, 200, pp. 296-300, (1992)

[29]

Pohl U., Ogilvie A., Lamontagne D., Busse R., Potent effects of AP<sub>3</sub>A and AP<sub>4</sub>A on coronary resistance and autacoid release of intact rabbit hearts, Am J Physiol, 260, pp. H1692-H1697, (1991)

[30]

Randerath K., Janeway C.M., Stephenson M.L., Zamecnik P.C., Isolation and characterization of dinucleoside tetraand triphosphates formed in the presence of lysyl-sRNA synthetase, Biochem Biophys Res Commun, 24, pp. 98-105, (1966)

← 1 2 3 4 →